Fig. 4. Ciliogenesis of isogenic LRRK2 G2019S+/- and its gene-corrected WT (Corr-WT) iPSC (A) and NSC (B) pairs. iPSCs were treated with DMSO (-) or 400 nM MLi-2 for 24 h (A) and NSC were exposed to B27 deprivation for 24 h to mimic serum starvation (B). Note that B27 deprivation could not remove all proteins because of other supplements in the medium. The treatment scheme, summary graph, and a representative image for each cell type are shown. Scale bar: 20μm. A total of 106~141 (A) or 75~201 cells (B) in two (A) or four (B) images were analyzed. The white arrow indicates a ciliated cell.
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