The information of the primary antibodies studied in this autopsy case
| Antibodies | Dilution | Clone | Antigen retrieval | Company |
|---|---|---|---|---|
| Aquaporin 4 | 1:2000 | Polyclonal | Ventana CC1 at 100°C | Millipore, Temecula, USA |
| CD3 | RTU | 2GV6 | Ventana CC1 at 100°C | Ventana |
| CD8 | RTU | SP57 | Ventana CC1 at 100°C | Ventana |
| CD20 | 1:500 | L26 | Ventana CC1 at 100°C | DAKO, Glostrup, Denmark |
| CD68 | 1:2000 | KP1 | Ventana CC1 at 100°C | DAKO, Glostrup, Denmark |
| CMV | 1:50 | CCH2+DDG9 | Ventana CC1 at 100°C | DAKO, Glostrup, Denmark |
| GFAP | RTU | EP672Y | Ventana CC1 at 100°C | Ventana |
| HSV | RTU | Polyclonal | Ventana CC1 at 100°C | DAKO, Glostrup, Denmark |
| IBA-1 | 1:12,000 | EPR16589 | Ventana CC1 at 100°C | ABCAM, Bristol, UK |
| NeuN | 1:500 | A60 | Ventana CC1 at 100°C | Millipore, Temecula, USA |
| NF | 1:2000 | 2F11 | Ventana CC1 at 100°C | DAKO, Glostrup, Denmark |
| Phosphorylated NF | 1:10,000 | NP-1 | Ventana CC1 at 100°C | Millipore, Temecula, USA |
| Synaptophysin | 1:200 | 27G12 | Ventana CC1 at 100°C | Novocastra, Newcastle, UK |
| α-synuclein | 1:1000 | EP1536Y | Ventana CC1 at 100°C | ABCAM, Bristol, UK |
| β-amyloid | 1:5000 | 6E10 | Ventana CC1 at 100°C | Biolegend, SanDiego, USA |
| 3 repeat (3R) tau | 1:200 | 8E6/C11 | Ventana CC1 at 100°C | Millipore, Temecula, USA |
| 4 repeat (4R) tau | 1:1000 | Polyclonal | Ventana CC1 at 100°C | Cosmobio, Tokyo, Japan |
| Phosphorylated-tau | 1:300 | AT8 | Ventana CC1 at 100°C | ThermoFisher, Waltham, USA |
| pTDP43 | 1:10,000 | pS409/410 | Ventana CC1 at 100°C | Cosmobio, Tokyo, Japan |
| TMEM119 | 1:500 | Polyclonal (c-ter) | Ventana CC1 at 100°C | ABCAM, Bristol, UK |
CMV, cytomegalovirus; GFAP, Glial fibrillary acidic protein; HSV, Herpes simplex virus; NeuN, Neuronal nuclei; NF, neurofilament; CD, Cluster of differentiation; p-Tau, phosphorylated-Tau; p-TDP43, Phosphorylated TAR DNA binding protein; RTU, ready to use.